PhysioMEA – Novel Open Source Platform for 1D and 2D Multi-Electrode Array Electrophysiological Data Analysis
PhysioMEA is a unique platform for analysis of multi-electrode array (MEA) data of excitable cell constructs and organoids, enhancing the field of cardiac electrophysiology. This platform includes an advanced toolbox and sophisticated graphical user interface (GUI) supporting the exploration of recorded data, segment selection, peak-detection, and biomarker calculation. Moreover, PhysioMEA enables, for the first time, comprehensive two-dimensional (2D) MEA analysis, including spatiotemporal biomarker heatmaps and Beat Rate Variability (BRV) analysis, with parameters adjusted to hiPSC-CM derived cardiac organoids. The platform may also support recordings from various sources, including cardiac structures, as well as neuronal tissues and organoids, enabling broad applications across the electrophysiological research area.
When using PhysioMEA resources, please reference the following paper:
Weiser-Bitoun Ido, Savyon Mazgaoker, Shani Assayag, Moran Davoodi, Alexandra Alexandrovich, and Yael Yaniv. “PhysioMEA: Signal processing platform for rate and rhythm analysis of multi-electrode array cardiac electrophysiological recordings.” Journal of Molecular and Cellular Cardiology (2025).
Video
PhysioMEA features include:
- Presentation of raw data from all measured electrodes
- Raw data filtration
- Peak Detection (R-, S-, and T-peaks) and Biomarker calculation (Inter Beat Interval-IBI, Amplitude-AMP, Peak-to-Peak Duration-PPD, Maximal Slope-Slope, Field Potential Duration-FPD)
- Beat Rate Variability (BRV) analysis including #### BRV measures (corresponding to Heart Rate Variability Analysis (HRV) for in-vitro derived data)
- Novel two-dimensional (2D) analysis of biomarkers as a figure or a video, presented solely by PhysioMEA
Contributors
The following individuals have contributed to the PhysioMEA project:
Prof. Yael Yaniv
Dr. Ido Weiser-Bitoun
Alexandra Alexandrovich
Dr. Moran Davoodi
Dr. Savyon Mazgaoker
Shani Assayag
Special Thanks: Assoc. Prof. Joachim A. Behar
License
Released under the GNU General Public License
This program is free software: you can redistribute it and/or modify it under the terms of the GNU General Public License as published by the Free Software Foundation, either version 3 of the License, or (at your option) any later version.
This program is distributed in the hope that it will be useful, but WITHOUT ANY WARRANTY; without even the implied warranty of MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the GNU General Public License for more details.
You should have received a copy of the GNU General Public License along with this program. If not, see http://www.gnu.org/licenses./
If you are a company and want to use PhysioMEA then please email us at: yaely@bm.technion.ac.il
Any use of the program or its resources should include a reference the mentioned above paper. Any commercial use of the program or its resources must get permission from the principal investigator.
Third-party attribution
PhysioMEA makes use of the following open-source software packages:
PhysioZoo
WFDB Toolbox for MATLAB
GUI Layout Toolbox
yamlmatlab
GetFullPath
matlab-jsystem
wavedet
Neuroshare Library
sigstar
Consult the project page of each above package for their respective license and copyright holder